Cell line authentication is achieved by genetic profiling using polymorphic short tandem repeat (STR) loci. STR loci consist of repetitive sequence elements 3–7 base pairs in length. These highly discriminative markers can be utilized using a rapid multiplex-PCR based method for the identification and detection of contaminating human cells.
We solve the issues associated with research involving cultured cell:
Cell misidentification and cross-contamination that are cause of data invalidation, lost of time, money and efforts.
Ensure to journals and funding agency good experimental practices in order to not fail journal and grant application.
We provide two panels for the authentication with validated kit.
Standard Analysis recommended from the American Tissue Culture Collection (ATCC) and published ASN-0002-2011, which includes 10 Human STR loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317, D5S818 and D21S11);
Contamination with mycoplasma is amongst the most notorious issues associated with cell cultivation. Depending on cell type, source and culture methods, the contamination with mycoplasma, acholeplasma and ureaplasma varies between 15 and 80%1. A contamination is not only inconvenient, but also a costly issue as it often requires the elimination of precious cultures.
Our service provide you a rapid and full answer.