Microbiome Test and Analysis
Stool - Buccal swab -Tears - Saliva - Environmental
Human & Veterinaries species
Fast, precise, cost effective
Stool - Buccal swab -Tears - Saliva - Environmental
Human & Veterinaries species
Fast, precise, cost effective
Microbiome testing NGS technology data available in less than 3 week
Labospace service team has tremendously simplified and increased the sequencing depth for 16S rRNA gene sequencing, by amplifying and sequencing seven hypervariable regions (V2, V3, V4, V6, V7, V8 and V9). We are equipped with the Ion Torrent™ PGene Studi S5 platform (ThermoFisher Scientific). One of the most commonly used and standardized platforms for metagenomics analysis of different microbiota.
Why our Microbiome test is different?
We do the test directly on DNA extracted from biological samples such as stool, , saliva, skin and swabs (vaginal, buccal, rectal, cutaneous or environmental).
Labospace metagenomic analysis allows qualitative and quantitative assessment of the bacteria (family, genus and species) present in the various anatomic districts in various health and / or pathological conditions.
Raw data can be provided:
1. As FASTQ or uBAM file
2. Analyzed with Ion Reporter™ software using MicroSEQ™ ID and Greengenes as reference databases
3. Analyzed with custom software that uses the RDP (Ribosomal Database Project) database
We can provide a data template report customized to meet specific research requirements, the base is supported by the raw data file generated and the preliminary analysis made through Ion Reporter™.
Report Example
Let us Know how we can help you
Consultation -Offered at no charge by our experts, it is essential to have an initial exchange regarding the sample type and general aims of the project. In order to effectively define and set clear objectives and outcomes.
Sample Collection and shipment – We will provide instructions on how to handle, process, and ship the sample, and specify the sample requirements (based on sample type). Collection kits are available for a prompt delivery. Collection kits are available for a prompt delivery.
Up and running:
Contact info@labospace.com and tell your needs
Discuss our proposal
SOW generation and approval
Fully organized logistic and samples pick-up
Data Delivery
References
Mathis D., Benoist C. (2011). Microbiota and autoimmune disease: the hosted self. Cell Host Microbe. 10, 297–30.
Hadis, U., Wahl, B., Schulz, O., Hardtke-Wolenski, M., Schippers, A., Wagner, N., Mu¨ ller, W., Sparwasser, T., Forster, R., and Pabst, O. (2011). Intestinal tolerance requires gut homing and expansion of FoxP3+ regulatory T cells in the lamina propria. Immunity 34, 237–246.
Littman D.R., Pamer E.G. (2011). Role of the Commensal Microbiota in Normal and Pathogenic Host Immune Responses. Cell Host Microbe. 10, 311–23.
Qin J, Li R, Raes J, Arumugam M, et. al. (2010). A human gut microbial gene catalogue established by metagenomic sequencing. Nature. (7285):59–65.
Ivanov I. I., Littman D. R. (2010). Segmented filamentous bacteria take the stage. Mucosal Immunol. 3, 209–212. doi: 10.1038/mi.2010.3.
Wu, H.J., Ivanov, I.I., Darce, J., Hattori, K., Shima, T., Umesaki, Y., Littman, D.R., Benoist, C., and Mathis, D. (2010). Gut-residing segmented filamentous bacteria drive autoimmune arthritis via T helper 17 cells . Immunity 32, 815–827.
Gaboriau-Routhiau, V., Rakotobe, S., Le´cuyer, E., Mulder, I., Lan, A., Bridonneau, C., Rochet, V., Pisi, A., De Paepe, M., Brandi, G., et al. (2009). The key role of segmented filamentous bacteria in the coordinated maturation of gut helper T cell responses. Immunity 31, 677–689.
For Research Use Only. Not for use in diagnostic procedures.
Our Delivery
Get your detailed bacteria map and full data
Our base reporting software enables the identification, at the genus or species level, of microbes present in complex polybacterial samples, and uses both the premium curated MicroSEQ™ ID 16S rRNA reference database and the curated Greengenes database.
We also provides primer information, classification information, percent ID, and mapping information. With easy-to-use interactive Krona chart displays, you can interpret population diversity for your research at any taxonomic level.
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